Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

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چکیده

This study aimed to investigate the effects of certain nutrient medium types enhanced with plant-growth regulators on direct inductionof somatic embryogenesis in vegetative strains produced and propagated from a tissue culture Digitalis purpurea. The results showthat MS was superior other tested media-B5, LS, 5C01—in terms days required initiate somaticembryogenesis induction rate. In contrast, LS resulted highest number embryos. Moreover, 2 mg/LBA (benzyl adenine) similar concentrations BAP aminopurine) Kin (kinetin) medium, whereasBAP performed better B5. On hand, auxin 2.4D (dichlorophenoxy acetic acid) inhibited embryogenesis, as didNAA (naphthalene IAA (indole when added individually. Regarding type explant, stems showedthe rates numbers direct-somatic embryos all basic media.Keywords: purpurea; Vegetative strains; Direct-somatic embryogenesis; Explant; Basic media

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ژورنال

عنوان ژورنال: Emirates Journal of Food and Agriculture

سال: 2023

ISSN: ['2079-0538', '2079-052X']

DOI: https://doi.org/10.9755/ejfa.2023.v35.i2.3008